2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

Electrokinetic Separation and Detection of DNA Nanoparticles and Cancer BioMarkers

M.J. Heller, R. Krishnan, B. Sullivan, R. Mifflin, S. Esener
University of California, San Diego, US

Keywords:
nanoparticles, electrokinetic, dielectrophoresis, cancer, DNA, biomarkers

Abstract:
In cancer research and diagnostics, it is a significant challenge to directly isolate and identify rare cancer cells and potential cancer markers such as high molecular weight DNA nanoparticulates and immunocomplexes in blood and plasma. Additionally, the advent of bionanotechnology has now led to numerous drug delivery approaches that involve encapsulation of drugs within nanovesicles and nanoparticles, which will also have to be identified and separated from blood and plasma. AC electrokinetic techniques such as dielectrophoresis (DEP) offer a particularly attractive mechanism for the separation of cells, biomarkers and drug delivery nanovesicles. Unfortunately, present DEP systems require significant dilution of the blood or plasma, thus making the technology less suitable for clinical sample preparation and diagnostic applications. Significant progress has now been made on using a DEP array system to separate and isolate cells, microspheres, and DNA nanoparticles under higher ionic strength conditions that more closely approach blood. More specifically, the separation of 60nm DNA nanoparticles into the DEP high field regions, and 10 micron microspheres into the DEP low field regions was carried in both 1X (89mM) TBE and 1X PBS (120mM). These buffer concentrations are from about one tenth to the equivalent conductance of blood and plasma.


Nanotech 2008 Conference Program Abstract