2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

Atomic Force/Scanning Probe Microscopy of Pharmaceutical and Biological Materials

D.A. Chernoff
Advanced Surface Microscopy, Inc., US

Keywords:
particle morphology, amorphous, crystalline, collagen, DNA, AFM, SPM

Abstract:
AFM is a valuable tool for a wide range of materials and device analysis. AFM images of particles and biopolymers are valued because direct visualization and measurement of individual objects complements the average measures obtained from bulk analysis techniques such as chromatography, spectroscopy and light scattering. The FDA encourages the use of AFM along with those techniques. Working with microspheres composed of an active drug compound dispersed in a polymer matrix, the maker found that particle flowability varied from lot to lot. AFM showed variations in the morphology of small drug particles on the microsphere surface. Lactose particles, long regarded as inert chemically and physically stable, may show changes in handling characteristics as they age. AFM showed molecular diffusion on the surface of a lactose single crystal, giving a mechanism for aggregate formation. AFM analyzes the molecular weight and conformation of collagen monomers by direct imaging of each molecule. Evaluating the length of individual collagen monomers and classifying the oligomers helps optimize the digestion and purification of collagen from animal tissue for subsequent use as a medical biomaterial. AFM images demonstrated a DNA-peptide complex which appears to be a key molecular player in psoriasis.


Nanotech 2008 Conference Program Abstract