2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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Clean Technology 2008

Adsorption kinetics of bacteria onto a thin Fe3O4 magnetic nanofilm

F. Ansari, A. Aref, S. Libor, R. Horvath, J.J. Ramsden
CRANFIELD University, UK

Keywords:
magnetic nanoparticles, monolayer, optical waveguide, adsorption, kinetics, bacteria

Abstract:
A range of bacteria have been shown to be able to remove sulfur from organic compounds that commonly exist in petroleum. The most extensively investigated microorganisms belong to the genus Rhodococcus, which has shown reasonably high activity and stability for removing sulfur from organic compounds, but this activity is unlikely to be sufficient for commercial applications. We found previously [1] that bacteria decorated with Fe3O4 magnetic nanoparticles had a higher desulfurization activity compared to the non-decorated cells, encouraging further development of bacteria for sulfur removal and designing new biodesulfurization processes. In order to investigate the stickiness of magnetic nanoparticles to the surface of bacteria, a thin layer of Fe3O4 magnetic nanoparticles was deposited onto a hydrophilic Si(Ti)O2 surface. The kinetics of adsorption of Rhodococcus erythropolis IGTS8 on the fabricated nanofilm was measured under controlled conditions using optical waveguide lightmode spectroscopy (OWLS) [2], with which the number of deposited particles and bacteria [3] could be accurately calculated.


Nanotech 2008 Conference Program Abstract