2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

Human monocyte uptake and carriage of IgG-coated nanoparticles to neuroinflamed brain subregions

A. B├ęduneau, H. Dou, C. Grotepas, M. Boska, N. Gong, A.V. Kabanov, H.E. Gendelman
University of Nebraska, US

Keywords:
cell-based delivery, monocytes, IgG coating, neuroinflammation

Abstract:
A cell-based delivery system was developed within our laboratories for the transport of superparamagnetic iron oxide nanoparticles (SPION) specifically to neuroinflamed brain subregions. Circulating monocytes effectively infiltrate sites of active neuroinflamation and as such were used for this strategy. Human immunoglobulin (IgG) was covalently attached to the surface of SPION to facilitate Fc-mediated uptake into human monocytes. The coupling efficiency was ~50% and the IgG-SPION were characterized by as size of ~175nm. The IgG coating increased SPION uptake into human monocytes without affecting cell viability. Histological and magnetic resonance imaging showed migration of human SPION-loaded monocytes into disease affected mouse brain tissue. These results have significant implications for cell-based carriage of drug-laden nanoparticles for delivery of drugs into the nervous system.


Nanotech 2008 Conference Program Abstract