2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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TechConnect Summit
Clean Technology 2008

Electrospun Nanofibrous Membrane Biosensor for lactate

J. Romero, I. Moreno, V. González
Centro deInvestigación en Química Aplicada, MX

Keywords:
lactate, bio-sensor, electrospinning, fibers

Abstract:
Electrospinning is a simple, non mechanical method used to produce non-woven fabrics of ultrafine fibers in the nanometer diameter range (nanofibers).This method is appropriated to obtain high-surface-area polymeric fibrous membranes and is a one-step process that do not require further treatment to induce porosity, such features have important advantages in applications (i.e. sensors and catalysts), where large surface areas are desired. On the other hand, there is considerable interest in measurements of blood lactate. While blood lactate is a useful diagnostic indicator, its use hindered by the time required for a lactate determination. In this work, fibers and non-woven mats of polyvinylpyrrolidone and beef heart lactate dehydrogenase (LDH) [previously noncovalently labeled with 8-anilino-1-naphthalene sulfonic acid (ANS)] solutions were prepared by electrospinning. Electrospinning configuration yielded fibers with cylindrical section and diameter ranging from 130 to 400 nm. ITO coated glasses were used as fiber collector to obtain non-woven mats, whose properties were evaluated by UV-Vis and photoluminescence spectroscopy. Non-wovens morphology and fiber diameter distribution were studied by scanning electron The decrease in emission intensity upon binding lactate to the noncovalent labeled LDH with ANS was used to measure the lactate concentration


Nanotech 2008 Conference Program Abstract