2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

Deconstructing Receptor Signaling with Nanopatterned Supported Membranes

K. Salaita, P. Nair, J.T. Groves
UC Berkeley, US

Keywords:
supported lipid bilayers, cell-surface signaling, RTK, diagnostics

Abstract:
Activation of the EphA2 receptor tyrosine kinase with its natively membrane-bound ligand, ephrin-A1, plays an important role in breast cancer biology. This talk examines the biological activity of the ephrin-A1 ligand as a function of how it is presented on a surface. Realtime fluorescence imaging, gene expression profiling, immunostaining, and western blots all show that ephrin-A1 is active when tethered to a fluid supported lipid membrane (SLB), and biological function is altered when presented in solution, tethered to a SLB in the gel-phase, or covalently immobilized onto a surface. The chemical composition of the surface is identical in all cases, but its two-dimensional mobility drastically alters biological function. Importantly, experiments reveal a mechanism by which nanoscale patterning may regulate cell signaling.


Nanotech 2008 Conference Program Abstract