Inhibition of Acetylcholine Esterase Promotes Growth of Silver Nanoparticles: Direct method to Sense Nerve Gases Using Nanotechnology
A. Virel, L.S. Peña, V. Pavlov
CIC Biomagune, ES
acetylcholine esterase, nanoparticles
Hydrolysis of acethylthiocholine (1) mediated by the acetylcholine esterase (2), AChE, yields thiol bearing compound thiocholine (3). The latter at trace concentration inhibits growth of silver nanoparticles on gold seeding nanoparticles (4) by the reduction of AgNO3 (5) with ascorbic acid (6). Very low concentrations of AChE inhibit formation of silver nanoparticles and catalytic growth of silver nanoparticles is promoted by inhibition of the enzyme by 1,5–bis(4-allyldimethylammoniumphenyl)-pentane-3-one dibromide (7) or by diethyl p-nitrophenyl phosphate (paraoxon; 8). Simple and sensitive colorimetric homogeneous and heterogeneous colorimetric assays for AChE inhibitors were developed.
Nanotech 2008 Conference Program Abstract