2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

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Clean Technology 2008

Landscape phage libraries as a source of bioselective nanomaterials

G.A. Kuzmicheva, I.B. Sorokulova, P.K. Jayanna, V.A. Petrenko
Auburn University, US

Keywords:
phage libraries, nanoparticles

Abstract:
New generations of targeted diagnostic, therapeutic and imaging nanodevices require robust molecular recognition interfaces, which bind their targets with high specificity and selectivity. These bioselective interfaces can be formed by recombinant filamentous phages or their isolated fusion proteins. Filamentous phages are nanoparticles with regular structure enable to display on their surface thousands of specific ligands. Such nanoparticles possess the essentially higher stabilities compared to specific antibodies, and could be kept for years without loosing their binding abilities. Based on the vector f8-6 harboring two conditionally lethal mutations in the gene gpVIII, we constructed a novel landscape peptide library, where random 9-mer peptides are presented as N’-terminal part of major coat protein pVIII and displayed on the phage surface in 4000 copies. The 9-mer library and 8-mer one constructed earlier (billions of primary clones each) have been used to select phages enable to bind proteins (streptavidin, TEM1 -lactamase,) and pathogenic bacteria Salmonella typhimurium. Selected phages were characterized by ELISA and co-precipitation assay. Phages selected from two libraries mostly have no overlapping or common motifs. Selected phages were used in phage based microarrays and biosensors. Constructed landscape phage libraries can serve as a universal source for novel stable bioselective nanomaterials with many applications in medicine and technology.


Nanotech 2008 Conference Program Abstract