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Real-time Detection and Quantitation of siRNAs in vivo
A. Cheng, Y. Liang, A. Vlassov, M. Li, Y. Wang, L. Wong, C. Chen, S. Magdaleno
Ambion Inc., An Applied Biosytems Business, US
Keywords:
RNAi, in vivo, siRNAs, detection
Abstract:
We have developed TaqMan based RT-PCR assays to detect and quantitate siRNAs in vivo. These assays can be used to measure siRNA delivery efficiency after injections and follow body distribution. These assays can also be used to understand the RNAi mechanism and pathways in vivo.
Nanotech 2008 Conference Program Abstract
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