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Subcellular fractionation in a fluidic microsystem
A. Paulus, A. Posch, M. Hausmann, G. Blankenstein, T. Duong, J. Kentsch, B. Angres, D. Stoll, M. Stelzle
NMI Natural and Medical Sciences Institute, DE
Keywords:
subcellular fractionation, biological nanoparticles, organelle, dielectrophoresis
Abstract:
The availability of an easy-to-use, reproducible method for subcellular fractionation would significantly promote qualitative and quantitative analysis of subcellular protein composition. A microfluidic system with integrated electrodes was designed and fabricated allowing for dielectrophoretic manipulation of biological nanoparticles. Fluorescently labelled mitochondria were successfully retrieved from a complex cell homogenate employing a dielectrophoretic band-bass electrode array. Dielectric properties of mitochondria were also investigated and biochemical analysis of fractions obtained was performed.
Nanotech 2008 Conference Program Abstract
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