2008 NSTI Nanotechnology Conference and Trade Show - Nanotech 2008 - 11th Annual

Partnering Events:

TechConnect Summit
Clean Technology 2008

Subcellular fractionation in a fluidic microsystem

A. Paulus, A. Posch, M. Hausmann, G. Blankenstein, T. Duong, J. Kentsch, B. Angres, D. Stoll, M. Stelzle
NMI Natural and Medical Sciences Institute, DE

subcellular fractionation, biological nanoparticles, organelle, dielectrophoresis

The availability of an easy-to-use, reproducible method for subcellular fractionation would significantly promote qualitative and quantitative analysis of subcellular protein composition. A microfluidic system with integrated electrodes was designed and fabricated allowing for dielectrophoretic manipulation of biological nanoparticles. Fluorescently labelled mitochondria were successfully retrieved from a complex cell homogenate employing a dielectrophoretic band-bass electrode array. Dielectric properties of mitochondria were also investigated and biochemical analysis of fractions obtained was performed.

Nanotech 2008 Conference Program Abstract