Chemically modified polacrylic acid monolayers in alumina nanoporous membranes for cancer detection
S.V. Atre, N. Monfared, M. Bothara, S. Prasad, S. Varadarajan
Oregon Nanoscience & Microtechnologies Insittute, US
polyacrylic acid, estradiol linker, nanoporous alumina, biosensor
We report the synthesis and modification of polyacrylic acid (PAA) monolayers in nanoporous alumina membranes. PAA was adsorbed onto the membrane surface using solution self-assembly to provide 0.5 -1 nm films that are robustly bound as carboxylate groups on the basic alumina surface. About 40% of the carboxylic acid groups are free to undego chemical reactions. Using amine linker chemistry and carbodiimide coupling, estradiol functionality was covaltently linked to the PAA monolayers chemisorbed on the nanoporous alumina. These sturctures were evaluated for binding to enzymes linked with breast cancer. Strategies and results for integrating on the surface of microelectrode array based chemirestitive biosensors are presented.
Nanotech 2008 Conference Program Abstract