2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

A method for the highly parallel analysis of gene expression of single cells

W.R.A. Meuleman, N. Milner, D.W.K. Lueerssen and E.M. Southern
Oxford Gene Technology, UK

Keywords:
DNA microarrays, hybridisation, single cell

Abstract:
Presented is a method for carrying out rapid single cell gene expression analyses on a large number of cells in parallel. The entire analysis is carried out on a DNA microarray. We demonstrate single molecule resolution without the use of PCR, allowing us to quantitatively analyse high as well as low levels of gene expression. Because of the parallel nature of the method, individual cells within a population can be compared rapidly and conveniently.

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