2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

DNA-DNA Interaction on Dendron-Functionalized Sol-Gel Silica Films Followed with Surface Plasmon Fluorescence Spectroscopy

S.H. Kwon, B.J. Hong, H.Y. Park, W. Knoll and J.W. Park
Center for Integrated Molecular System., KR

Keywords:
dendron, self-assembly, DNA hybridization, kinetics, SPFS, sol-gel

Abstract:
Since we observed that dendron-assembled surface providing a proper spacing between immobilized DNAs showed high single nucleotide polymorphism discrimination efficiency for DNA microarrays(1), the nanoscale-controlled surface is examined with surface plasmon field-enhanced fluorescence spectroscopy (or SPFS) to elucidate the kinetics of the DNA hybridization. A silica film (d=33 nm) was coated onto a gold surface using the sol-gel technique to suppress fluorescence quenching, followed by self-assembly of a second-generation dendron and covalent immobilization of a catcher DNA strand at the dendron’s apex. The association rate for the DNA hybridization on the surface was faster than that reported for a streptavidin-modified surface. (2) In addition, we observed that the capability to capture DNA target strands was maintained even after extended storage at ambient conditions.

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