2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

Quantum Dot Labeling of Retinal Tissue Sections

C.W. Sharp, S. Pathak, M. Davidson and G.A. Silva
Univeristy of California, San Diego, US

Keywords:
QDot, astrocyte, reactive gliosis, retina

Abstract:
Quantum dot labeling is a new tool of significant potential for neuroscience research and may have unique applications for studying reactive gliosis, a ubiquitous neuropathological process. Since quantum dots exhibit stable fluorescent optical properties and can be attached to different proteins expressed by cells within the nervous system, they provide a promising alternative to traditional immunocytochemistry techniques for studying and imaging the anatomy and histology of the retina. By labeling retinal tissue sections (~5 um thick) with quantum dots, we have been able to explore the interactions between different cell types in the retina, including glial cells, in detail with high spatial resolution.

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