2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

Photo-induced reduction of catalytically and biologically active Ru(II)bisterpyridine-cytochrome c bioconjugates

J.R. Peterson, T.A. Smith and P. Thordarson
The University of Sydney, AU

Keywords:
ruthenium, terpyridine, cytochrome, bioelectronics, bioconjugates

Abstract:
The objective of this work is to synthesize catalytically and biologically active bioconjugates that could be incorporated into bioelectronic circuits and sensors that are responsive to light. To that end, synthesized Ru(II)-bisterpyridine chromophores were attached to iso-1-cytochrome c from saccharomyces cerevisiae using two different spacer molecules to form light activated donor-acceptor bioconjugates. We show that the resulting bioconjugates are catalytically and biologically active, and provide evidence of photo-induced electron transfer from the Ru(II)-bisterpyridine donor to the cytochrome c acceptor.

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