2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

Efficient gene and protein delivery using superparamagnetic nanoparticles

S.W. Kamau, P.O. Hassa, B. Steitz, A. Petri-Fink, H. Hofman, M. Hofman, B. von Rechenberg and M.O. Hottiger
University of Zurich, CH

Keywords:
superparamagnetic nanoparticles, gene and protein delivery

Abstract:
Superparamagnetic nanoparticles (SPIONs) based on iron oxide, have an advantage in gene and protein delivery since their combination with magnets that can draw particles to a desired location and keep them at a specific site. We explore the use of polyethylenimine (PEI) coated SPIONs, for delivery of novel shorter DNA fragments (PCR products), plasmids and purified green fluorescent protein (GFP) in presence of a magnetic field. Our results show that PEI coated SPIONs were effective delivery vectors for gene and protein. The gene delivery rates achieved with SPIONs within 5 min were significantly higher than those achieved with conventional transfection methods. This was also observed when novel PCR products containing only the sequence of the gene of interest, were used. The application of a pulsating magnetic field, after sedimentation of the particles with a static magnetic field increased the transfection efficiency significantly, over the magnetofection. PEI coated SPIONs were also very efficient in delivery of purified His-tagged GFP, within 5 minutes of exposure. In conclusion, we show that PEI coated SPIONs are effective delivery vectors for PCR products which are attractive alternatives to plasmids, as well as purified proteins. The application of a pulsating magnetic field enhances the already high gene delivery in presence of static magnet.

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