2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

Development of a specific micro/nanodevice validating the concept of molecular biopsy

F. Berger, A. Bouamrani, M. Cosnier, F. Martin, P. Caillat and A.L. Benabid
Inserm, FR

Keywords:
Proteins, surface modification, silicon chip

Abstract:
The nanomedicine concept implies early detection at the molecular level and molecular characterisation to implement a specific therapy. In this context, the development of micro/nanotool devoted for intra-tissular characterisation is indispensable. We first demonstrated that proteins can be captured from the microsurgery tools directly introduced in the human brain. We optimized the tool by the addition of specific chemical and micro/nanostructuration modifications. A small silicon chip was produced, and plugged on the metallic stylet. We first introduced chemical modification on the surface, mimicking in vivo the SELDI-TOF concept, devoted to the capture of a specific subproteome. The results obtained clearly demonstrate the adequate concentration of a specific subproteome providing the individualization of supplementary peptides. The chemical modifications also provided the conservation of the spatial location of the proteins inside the tissue. We also significantly increased protein capture by the addition of specific micro/nanostructuration modifications on the surface of the device. In conclusion, we validate the concept of molecular biopsy using a specific silicon chip harboring surface modifications. This will be helpful, to help diagnosis, prognosis and therapeutic prediction during conventional micro-invasive approaches in oncology in connection with poly-omic characterization.

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Nanotech 2007 Conference Program Abstract

 
 

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