2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

Interaction of Nanoparticles with Normal and Cancer Breast Cells

C. Ozkan, Y. Zhang, V. Yazdanpanah, C.Y. Liu, S. Andavan, N. Portney and M. Ozkan
University of California, Riverside, US

Keywords:
nanoparticles, scanning probe microscopy, cancer cells

Abstract:
The mechanism of recognition and capture of superparamagnetic nanoparticles by specific cells and tissues is crucial to their future biomedical applications. We report the interaction of normal breast cells, MCF10A, and cancer breast cells, MCF7 with iron oxide nanoparticles incubated for different time periods using a hybrid near field scanning optical microscopy/atomic force microscopy (NSOM/AFM) system. By using this hybrid system, we could simultaneous record surface topography and the internal morphological information of cells. The resolution is observed to be very close to the size of imaging tip. Both tapping or contact AFM modes with transmission or reflection NSOM modes are used to visualize the iron oxide nanoparticles in interaction with both normal and cancer breast cells for uptake. Endocytosis mechanism was observed to be a two-step process including binding at the cell membrane first, followed by subsequent internalization. Next, we will describe an interaction study between iron oxide nanoparticles with normal breast cells (MCF10A) and cancer breast cells (MCF7) by investigating surface charge change using zeta potential measurements. We incubated the nanoparticles with normal and cancer cells for different time intervals. Significant surface charge changes for different incubation times with different nanoparticles were measured. Both types of cells are confirmed to uptake actively iron oxide nanoparticles via observations using transmission electron microscopy and near field scanning optical microscopy.

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Nanotech 2007 Conference Program Abstract

 
 

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