2007 NSTI Nanotechnology Conference and Trade Show - Nanotech 2007 - 10th Annual

Differential Cytotoxicity of Metal Oxide Nanoparticles

J. Chen, J. Zhu, H.-H. Cho, K. Cui, F. Li, X. Zhou, J.T. Rogers, S.T.C. Wong and X. Huang
Brigham and Women's Hospital, Harvard Medical School, US

Keywords:
metal oxide nanoparticle, high-content cell imaging, CNS, cytotoxicity, cell death, SH-SY5Y neuroblastoma cell, H4 neuroglioma cell

Abstract:
There are growing concerns among the general public and regulators about their potential health hazard. To determine the potentially toxic responses of CNS due to exposure of metal oxide nanoparticles, human SH-SY5Y neuroblastoma and H4 neuroglioma cells from the ATCC (Manassas, VA) were employed as cell models. The cells were incubated for 48 hours under the cell culture conditions (95% O2, 5% CO2, 85% humidity, 37ºC), together with Fe2O3, CuO, and ZnO nanoparticles and their metal ion counterparts (Fe3+, Cu2+, and Zn2+) at a concentration range of 0.01-100 µM. Their ensemble cell viability was determined using colormetry-based LDH and MTS cell proliferation assays. The nanoparticles and their counterpart metal ions had a similar toxicity profiles while CuO nanoparticles and Cu2+ attenuated the cell viability the most. To further quantify cytotoxic effects of the nanoparticles, a live/dead cell assay was also performed. Cellular images were acquired by a high-content and automatic fluorescence microscopy- In Cell Analyzer 1000 (GE Healthcare) with a 10X objective amplification. The images were further subjected to a novel computerized image analysis protocol developed by us. Our data indicated that CuO nanoparticles engendered significant cell death in both H4 and SH-SY5Y cells (more strikingly) while cytotoxic effects of Fe2O3 and ZnO nanoparticles were marginal compared to controls. These results were in good agreement with our ensemble cellular activity measurements. In conclusion, both H4 and SH-SY5Y cells had dose-dependent toxic responses to the insult of CuO nanoparticles.

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