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Optimizing antibody microarrays for high resolution SPR microscopy

G. Kim, A. Nishimoto, S. Cong, E. Johnson, H.W. Guan, B. Petcavish, S. Lasky, L. Hood and C.T. Campbell
Lumera Corporation, US

Keywords:
immunoassay, spr, protein microarray, biosensor

Abstract:
A high resolution surface plasmon resonance (SPR) microscopy technique based on the combination of Lumera’s polymer and proprietary optic technologies offers parallel high-throughput analysis of biomolecular binding kinetics on high-density antibody microarrays ( > 1,500 spots in 1.4 cm2 ). The high resolution SPR microscopy also shows potential as a quantitative drug screening tool (Binding kinetics of a 1500 Dal. peptide has been experimentally demonstrated). However, the high resolution SPR microscopy requires optimal surface modification and dense sample addressing technology. Another constraint for the high density biomolecular microarray experiment is maintaining the activity of proteins during the microarray fabrication process. In this report, various spotting solutions combined with different surface modification chemistries are evaluated for the uniformity and integrity of protein spots in microarrays. The effects of various spotting solution compositions on the protein activity resulting from the microarray fabrication process are also investigated

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