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Assessment of the dielectric properties of drug sensitive and resistant leukaemic cells before and after ion channel blockers using dielectrophoresis

L. Duncan, H. Shelmerdine, H.M. Coley and F.H. Labeed
University of Surrey, UK

Keywords:
ion channel, blocker, conductivity, dielectrophoresis

Abstract:
Dielectrophoresis (DEP) is a phemomenon of induced particle motion in non-uniform electric fields. The effect is frequency dependent; by monitoring the motion of particles in AC fields and ananlysing the change in motion with frequency, it is possible to determine the electrical properties of single cells in lab-on-a-chip systems. In this paper we use DEP to study the electrical properties of drug-sensitive and drug-resistant cancer cells before and after treatment with channel blocking agents, which give insight into the origin of cytoplasmic differences shown to have a significant bearing on the origin of drug resistance in cancer.

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