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New Guidelines for Selecting Best Extraction Methods of EPS Using Atomic Force Microscopy

S. Kang, Y. Ahn, H. Choi and M. Elimelech
Yale University, US

Keywords:
atomic force microscopy, extracellular polymeric substances, extraction, bio-probe, steric model

Abstract:
In this study, we applied AFM to develop the guideline during the selection of best extraction methods for exocellular polymeric substances (EPS). A single cell of S. cerevisiae was glued at the end of the tipless cantilever and forces were measured to measure the thickness of EPS layers as well as contour length of cellular polymers. A new analytical methods was very effective and gave consistant results with classical guidelines.Considering results, atomic force microscopy could be applied to develop, or to investigate the new and existing analytical methods in the field of environmental science and engineering.

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