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Study of Bioactive Films Using Natural Macromolecules

M. Ferreira, V. Zucolotto, M. Mulato and O.N. Oliveira Jr
FFCLRP-USP, BR

Keywords:
layer-by-layer, natural macromolecules, bioactive films

Abstract:
The control of interactions between living cells and modified surfaces is important for tissue engineering and may assist in the fabrication of bioactive coatings. Here we investigated the attachment of fibroblasts cells on bio-surfaces fabricated with humic acid and natural rubber. We have produced LbL films with up to 10 bilayers of PAH/HA or PAH/(HA+Latex) deposited directly on tissue culture polystyrene substrates (TCPS). In control experiments, we obtained cast films on TCPS substrates using fresh latex and 0.5 g/L solutions of HA in different proportions (latex:HA = 10:1, 5:1, 1:1, 1:2). Human fibroblast cells were cultured on the TCPS using the specific medium. An optical microscope (10 x magnification) was used to take images 1, 2 and 4 days after the cell incubation. Both PAH/HA and PAH/(HA+Latex) LbL films exhibited a good performance on cell adhesion and growth. On the other hand, the cast films from fresh latex:HA mixture were cell resistant surfaces and bioinert coatings. Figures 1.a-c and 2.a-c show the phase contrast micrographs of human fibroblasts cells acquired after 4 days of seeding at 10 000 cells/cm2. These data demonstrate the viability of creating cell interactive materials from natural macromolecules using the LbL technique.

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