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Fast and Efficient Nucleic Acid Testing by ST‘s In-CheckTM Lab-on-Chip Platform

S. Conoci, P. Di Pietro, S. Petralia, M.G. Amore, F. San Biagio, G. Alaimo, G. Iacono, E. Alessi, D. Ricceri, G. Di Trapani, F. Di Francesco and M. Palmieri
STMicroelectronics, IT

Keywords:
lab-on-chip, integration, PCR, microarray

Abstract:
Nucleic Acid Testing (NAT) represents one the most sensitive and effective method for several applications in the molecular diagnostic area, including clinical, industrial, veterinary and research applications. In this context, the development of miniaturized devices can offer relevant advantages in terms of performances (sensitivity, reliability), cost and speed.
In this contribution we report some examples of NAT analyses by using the ST’s In-Check™ lab-on-chip Platform (fig.1). This latter is based on a miniaturized silicon chip (LoC) that integrates a Polymerase Chain Reaction (PCR) reactor - formed by buried microchannels 1 ul in volume – together with a customable microarray. The Temperature Control System (TCS) and the Optical Reader (OR) appliances, driven by a tailored bioinformatic system, complete the platform. Therefore, these features allow ST’s In-Check™ lab-on-chip Platform to achieve both DNA amplification and NAT analysis in an integrated cost effective and convenient format silicon chip.

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