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Glycosaminoglycan model glass substrates and cancer cell interactions

A. Peramo, G. Wright, M.B. Meads, W.S. Dalton and G. Matthews
University of South Florida, US

Keywords:
glycosaminoglycan, cancer, surface, biopolymer, proteoglycan

Abstract:
The static adhesion of three cancer cell lines to surfaces functionalized with glycosaminoglycans was performed and the possible involvement of heparanase in this process was analyzed. Four different glycosaminoglycans (heparan sulfate, chondroitin sulfate A, chondroitin sulfate C and keratan sulfate) were deposited on glass surfaces that had been previously coated with 3-aminopropyltriethoxy-silane (APTES), and the substrates were used in static adhesion experiments. BT20, a moderately metastatic breast cancer cell line, MCF7 a non-metastatic breast cancer cell line, and A431, a highly metastatic epidermoid skin carcinoma cell line, were selected because of their different metastatic activity.
 
Glycosaminoglycans were covalently bonded to previously silanized glass cover slips. The molecular structure of the functionalized surface can be understood as a reduced section of the outer areas of the extracellular glycocalyx or the ECM containing proteoglycans.
 
The significance of the work is that it permits physical characterization of cancer-cell surface interactions by using in vitro model surfaces containing molecular species of interest with the possibility of selecting the molecular composition at the interface. In this case, with a group of polysaccharides present in several tissues of mammals.

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