Nanotechnology Conference and Trade Show - Nanotech 2006
> Program > Technical Conferences > Business & Development > Nano Impact Workshop > Nanotech Job Fair > Expo
Program
Sessions
Sunday
Monday
Tuesday
Wednesday
Thursday
Index of Authors
Index of Keywords
Confirmed Speakers
Conferences & Symposia

Conference Proceedings

Conference Technical Proceedings

Enhancement of Oxygen Transfer in Fermentation by Use of Functionalized Magnetic Nanoparticles

B. Olle, L. Bromberg, T.A. Hatton and D.I.C. Wang
Massachusetts Institute of Technology, US

Keywords:
nanoparticle, mass transfer, oxygen, enhancement, magnetic fluids, fermentation, gas absorption

Abstract:
Maintaining an adequate oxygen supply to aerobic cell cultures has been a long-standing problem in fermentation technology. Insufficient oxygen transfer rates limit cell growth and ultimately process productivity. Our approach to reducing the oxygen transport limitation consists in adding functionalized magnetic nanoparticles to the fermentation medium; these materials consist of particles that have a magnetic core and two coatings. The magnetic core facilitates recovery of the fluid after the fermentation by passing it through a magnetic field; the first coating, made of oleic acid, confers high-oxygen storing capacity and the outer coating, made of surfactant, confers colloidal stability in water to the particle. Using magnetic nanoparticles presents several advantages compared to previous approaches, including large interfacial areas and the possibility to readily recover the particles by High-Gradient Magnetic Separation.
An increase of 40% in the oxygen uptake rate has been achieved in Escherichia Coli fermentation by using 0.6% w/w particles, which is directly translatable into increased fermenter productivity. Oxygen transfer enhancements of up to 600% have been observed in cell-free media. It has also been shown that (i) both the mass transfer coefficient (kL) and the gas-liquid interfacial area (a) are enhanced in the presence of nanoparticles, (ii) the enhancement in kL levels off at a nanoparticle fraction of approximately 1% w/w, and (iv) the enhancement shows a strong temperature dependence.

Back to Program

Sessions Sunday Monday Tuesday Wednesday Thursday Authors

Nanotech 2006 Conference Program Abstract

 
Nanotechnology Conference | Terms of use | Privacy policy | Contact | NSTI Home
Program | Technical Conferences | Business & Development | Nano Impact Workshop | Nanotech Job Fair | Expo |
Nanotech 2006 Home | Press Room | Venue | Subscribe | Site Map
Names, and logos of other organizations are the property of those organizations and not of NSTI.
This event is not open to the general public and NSTI reserves the right to refuse admission and participation to any individual.