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Nano-complexes of Liposome Encapsulated Nanocrystals

I. Mandelbaum, P. Yim, J. Hwang, A.R. Hight Walker
NIST, US

Keywords:
nanocrystals, liposomes, quantum dots, fluorescence

Abstract:
We will present techniques to encapsulate NCs with different surface functional coatings within approximately 100nm unilamellar liposomes prepared by a variety of methods such as injection, inverted micells, and elecro-formation. Fluorescent lipid analogs were used to label liposomes to verify the encapsulation by assessing fluorescence resonance energy transfer between the donors (NCs) and the acceptors (lipid analogs) in total internal reflection fluorescence microscopy (TIRFM). The TIRFM allows us to excite only liposomes near the substrate surface therefore substantially enhances the signal to noise ratio in detection. We will also discuss the characteristics of each component, liposomes and functionalized NCs, and their complexes from results obtained by other methods including polarization modulation optical microscopy, and electron microscopy.

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