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A Capillary Electrophoresis Chip for Rhodamine B and Inorganic Ion detection Utilizing Contactless Capacitance Detection

W.L. Liu, W.J. Chen, S.H. Hsieh and T.K. Tsai
National Pingtung University of Science and Technology, TW

Keywords:
capillary electrophoresis, contactless capacitance detector, microchip, microfluid electrophoresis

Abstract:
A microfluidic channel and a contactless capacitance detector were
integrated on a glass chip by a semiconductor manufacturing process for
separating and analyzing Rhodamine B and inorganic ion. The electrodes of the
capacitance detector were formed successively by sputtering, electroless and electrodepositions. Samples containing fluorescent agent (Rhodamine B) and inorganic ions (K+, Na+, and Li+) were injected and separated in the microfluidic channel by the electrical voltage and detected by contactless capacitance measurement proposed in this work. The results showed that all the measured capacitance signals were very sharp and the reproducibility was quite well.

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