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High-Throughput Screening Devices Based on Hydrophobic Microfluidics

J.J. Chen, H.J. Yang, J.W. Wu, S.H. Parng and T.M. Yu
Industrial Technology Research Institute, TW

Keywords:
high-throughput screening, evenly distributed flow, hydrophobic valve

Abstract:
In this work, we developed a microfluidic assay for determination of the individual cell’s response to chemical exposure. Microfluidic chips comprisd four channels were fabricated on polystyrene (PS) substrates via mechanical milling processes, and covered by polydimethylsiloxane (PDMS) plates. This chip was designed to provide an evenly distributed flow to each perfusion channel and to assure the success of the liquid filling processes. The fabricated chips were characterized by using commercial ink and/or distilled water to see the bubbles progressing along the channels. The blue ink was injected into the bubbles to demonstrate the high-throughput drug screening process. All the blue ink slugs progressed down the channels successfully without any dilutions with the liquid water. In the future, this apparatus will serve as a tool for biochemical analyses.

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