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Spontaneously Forming Unilamellar Nano-Sized Vesicles – Polydispersity, Size, Shape and Stability

M-P Nieh, V.A. Raghunathan, C-Y Huang, J. Pencer, T. Harroun and J. Katsaras
National Research Council at Canada, CA

Keywords:
liposome, Unilamellar vesicles, spontaneous forming, phospholipid, neutron scattering, stability, low polydispersity

Abstract:
Unilamellar vesicles (ULV) made up entirely of biologically relevant materials can serve as carriers for drug delivery or gene therapy. Compared to extrusion and sonication methods, traditionally used for producing ULV, spontaneously forming ULV offer a more cost-effective method for their mass production. Moreover, the fact that these ULV are most likely thermodynamically stable assures that the product will have an extended shelf life. Here we report recent work on a phospholipid mixture of long- and short-chain lipids (i.e., di 14:0 and di 6:0 phosphatidylcholine), and we address some of the important factors that affect the polydispersity, size, shape and stability of these ULV.

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