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Nano-approach for sensitive monitoring of bacteria

S. Ignatov, S. Virjasov, G. Fedjukina, E. Baranova, S. Biketov
State Research Center of Applied Microbiology and Biotechnolog, RU

Keywords:
bacteria, identification, pathogen, antibodies, protein A

Abstract:
The sensitive method for pathogen identification is very important in medicine, food analysis, and environmental monitoring. The unique ability of AFM to image bacterial world at subnanometer resolution and in aqueous solution was opened new fields to investigate bacteria themselves and their surfaces under physiological conditions without any type of labeling. In the present work, we attempt to identify pathogen bacteria Legionella pneumophilla and bacterial spores of Bacillus thurengiensis with using AFM. For specific bacterial visualization the new approach based on immobilization of specific antibodies indirectly to the solid surface via antibody-binding staphylococcal protein A was developed. Bacterial cells and spores have been chosen to discriminate objects on AFM pictures by forms and sizes in the presence contaminates. The layer of antibodies against bacteria was exposed with bacteria and to evaluate the specificity with spores. The layer of antibodies against spores was exposed with spores and bacteria too. The AFM pictures were analyzed on the presence bacterial cells or spores.

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