Nanotechnology Conference and Trade Show - Nanotech 2006
> Program > Technical Conferences > Business & Development > Nano Impact Workshop > Nanotech Job Fair > Expo
Program
Sessions
Sunday
Monday
Tuesday
Wednesday
Thursday
Index of Authors
Index of Keywords
Confirmed Speakers
Conferences & Symposia

Conference Proceedings

Conference Technical Proceedings

Construction of Enzymosomes: Optimization of Coupling Parameters

C.A. Vale, M.L. Corvo, L.C.D. Martins, C.R. Marques, G. Storm, M.E.M. Cruz and M.B.F. Martins
Laboratorio Nacional de Engenharia, Tecnologia e Inovacao, IP, PT

Keywords:
enzymosomes, bioconjugates, lipid vesicles, therapeutic proteins, protein delivery

Abstract:
Different strategies can be used to improve carrier mediated delivery of therapeutic proteins. The incorporation of therapeutic enzymes into polymeric carriers [1], into aqueous space of lipid vesicles [2] or lipid-detergent vesicles [3, 4] or incorporation of hydrophobized forms into lipid bilayer of vesicles [5, 6, 7] are examples.
Another strategy, not usually used for therapeutic enzymes, is its attachment to the outer surface of liposomes, using technologies developed for antibodies [8, 9]. Few publications report the construction of carriers with surface attached enzymes [10] in contrast with a huge number of publications with antibodies attached to carriers, a concept widely used for the active targeting of nanocarriers.
In this work, we optimized processes to link covalently enzymes to the surface of lipid carriers, based on techniques of attachment of antibodies to liposomes. Our goal and concept is to build enzymosomes, as a tailor made load of therapeutic enzymes to liposome surface, to modulate the enzyme therapeutic effect by optimized carrier mediated transport. We achieve preservation of enzyme activity and preservation of vesicles structural integrity, as shown by in vivo fate.
The increasing number of potentially active proteins and its particular structural complexity are reasons for significance of this work to the drug delivery field.

Back to Program

Sessions Sunday Monday Tuesday Wednesday Thursday Authors

Nanotech 2006 Conference Program Abstract

 
Nanotechnology Conference | Terms of use | Privacy policy | Contact | NSTI Home
Program | Technical Conferences | Business & Development | Nano Impact Workshop | Nanotech Job Fair | Expo |
Nanotech 2006 Home | Press Room | Venue | Subscribe | Site Map
Names, and logos of other organizations are the property of those organizations and not of NSTI.
This event is not open to the general public and NSTI reserves the right to refuse admission and participation to any individual.