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Nanofluidic Size Exclusion Chromatograph for In Site Macromolecular analyses

D. Choi, F. Grunthaner, S. Feldman and D. Svehla
Jet Propulsion Laboratory/California Institute of Technology, US

Keywords:
nanofluidic, lab-on-a-chip, chromatograph, separation

Abstract:
We are developing a nanofluidic size exclusion chromatograph (nano-SEC) system that can be incorporated into miniature lab-on-a-chip devices which will provide detailed information on the geochemical and biological history of soil, ice, and water samples by identifying and qualifying a broad range of macromolecular polar and nonpolar organic compounds. Molecules traveling through a microcolumn containing nanometer-scale features will have characteristic elution times that directly correlate to molecular weight. Advantages of our nano-SEC over conventional SEC are expected to include the following: 1) column reproducibility is greatly aided by the microfabrication process; 2) column packing is not required; 3) minute sample quantities with sub-nl injection volumes can be analyzed; 4) greater control over size exclusion separation parameters can be achieved; 5) the complete nano-SEC system is expected to be portable, robust, miniature, and operate on low voltages and low powers.

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