The Development of a Microscale Cell Culture Analog Device to Probe Naphthalene Toxicity
X. Li, K. Viravaidya and M.L. Shuler
Cornell University, US
microscale cell culture analog, naphthalene, toxicity
Cells grown on microscale chips can be used to evaluate the effects of drugs and chemicals at an early stage, at molecular and cellular levels. Such devices are called microscale cell culture analog (_CCA). In this work, a four-chamber (lung-liver-fat-other tissue) _CCA system containing two-dimensional L2 rat lung cells, HepG2/C3A human hepatocytes and differentiated 3T3-L1 preadipocytes cells is treated with naphthalene or different naphthalenediols for 6 hours and the toxicity is studied. New generations of _CCA systems with cells cultured three-dimensionally in gels (Matrigel, Collagen, Alginate, Agarose or Gelatin) have also been developed. In three-dimensional cell cultures, the biological environment of real tissues can be better reproduced. Compared with animal experiments, this new technology is faster, cheaper, more efficient and much less complicated in result interpretation.
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