Microfabricated Silicon Apertures for Ion Channel Measurement
S.J. Wilk, M. Goryll, L. Petrossian, G.M. Laws, S.M. Goodnick, T.J. Thornton, M. Saraniti, J.M. Tang and R.S. Eisenberg
Arizona State University, US
ion channels, microfabrication, membranes
Ion channels are proteins that act as pores in cell membranes and allow only specific ions to pass through the cell wall. Here, we present a method for microfabrication of apertures in a silicon substrate using well-known cleanroom technologies. Apertures were etched through a silicon wafer using a plasma-etcher and then rendered hydrophobic with chemical vapor deposition of a Teflon surface layer. Characteristic measurements showing physiological single channel gating behavior of OmpF porin and alpha-hemolysin channel proteins have been made.
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Nanotech 2005 Conference Program Abstract