Nano Science and Technology InstituteNano Science and Technology Institute
Nano Science and Technology Institute 2005 NSTI Nanotechnology Conference & Trade Show
Nanotech 2005
Bio Nano 2005
Business & Investment
Nano Impact Workshop
Program
Sessions
Sunday
Monday
Tuesday
Wednesday
Thursday
Index of Authors
Index of Keywords
Keynote Presentations
Confirmed Speakers
Participating Companies
Industry Focus Sessions
Nanotech Expo
Special Symposia
Conferences
Sponsors
Exhibitors
Venue 2005
Organization
Press Room
Subscribe
Site Map
Nanotech 2005 At A Glance
Nanotech Proceedings
Nanotechnology Proceedings
Global Partner
nano tech
Supporting Organizations
Nanotech 2005 Supporting Organization
Media Sponsors
Nanotech 2005 Medias Sponsors
Event Contact
696 San Ramon Valley Blvd., Ste. 423
Danville, CA 94526
Ph: (925) 353-5004
Fx: (925) 886-8461
E-mail:
 
 

A Demonstration of High-throughput Immunoassay and Small Molecule Binding on Protein Microarrays with SPR Microscopy

G. Kim, L. Jiang, P.K. Rathod, C.T. Campbell, A. Nishimoto and V. Casasanta
University of Washington, US

Keywords:
immunoassay, spr, protein microarray, biosensor

Abstract:
The use of Surface Plasmon Resonance (SPR) for the detection of biomolecular binding events at the nanometer scale is of great utility when expanded into microarray density formats. Protein microarray analysis is particularly well suited for such methodology. In this work we demonstrate a high-throughput, quantitative immunoassay by employing microarray techniques in combination with SPR microscopy. Antibody microarrays with various immunoglobulin proteins, including human immunoglobulin subclasses were printed on functionalized gold-coated glass slides. Gold-coated glass slides presenting amine reactive moieties were prepared by traditional silation reactions to facilitate protein microarray printing. Antigen microarrays were also printed with various allergens including human serum albumin as well as bovine serum albumin in similar fashion. A spot density of up to 240 120-µm spots was generated using conventional robotic microspotting. Application of antibody/antigen microarrays with SPR microscopy allows for the simultaneous monitoring of immunoglobulin protein binding kinetics over the whole array (allergens or antibodies), at a time resolution of one second. The ability of microarray based SPR to quantitatively monitor (in “real-time”) antigen-antibody binding kinetics allows it to become a complementary tool in support of the multi-step and labor-intensive ELISA method (with a sensitivity of 1.2 ng/cm2 for most proteins).

Back to Program

Sessions Sunday Monday Tuesday Wednesday Thursday Authors

Nanotech 2005 Conference Program Abstract

 
Gold Sponsors
Nanotech Gold Sponsors
Silver Sponsors
Nanotech Silver Sponsors
Gold Key Sponsors
Nanotech Gold Key Sponsors
Nanotech Ventures Sponsors
Nanotech Ventures Sponsors
Sponsors
Nanotech Sponsors
News Headlines
NSTI Online Community
 
 

© Nano Science and Technology Institute, all rights reserved.
Terms of use | Privacy policy | Contact