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Nanomagnetic Immunoassay for Rapid Detection of Escherichia coli

Y. Haik, A. Qablan and C-J Chen
Center for Nanomagnetics and Biotechnology, US

Keywords:
nanomagnetic, immunoassay, E.Coli

Abstract:
This study identifies a simple process to quickly conduct an analysis, under field conditions, for detection of pathogens. A magnetic sandwich immunoassay is developed for rapid isolation and detection of targeted pathogen. Escherichia coli BL21 Star (DE3) pLysS is used as a model in this study. The magnetic immunoassay takes advantage of the multiple epitopes that are found on the target. On one epitope a nanomagnetic particle is attached. On another epitope a glucose molecule is attached. The sandwich magnetic assay that contains the glucose label can be easily correlated to the concentration of the Escherichia coli BL21 Star (DE3) pLysS.

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