New Light Induced Molecular Switch allows Sterically Oriented Micrometer Sized Immobilization of Biomolecules

Keywords:
biosensor, UV, protein, immobilization, nanoarray

Abstract:
Fundamental knowledge on protein structures and the effect of ultraviolet light on these structures has paved the way for the development of a unique light-based immobilization technology that allows oriented protein immobilization onto micrometer sized spots. The methodology is considered to be a strong alternative to the conventional procedures which often include the use of harsh conditions such as strong chemicals and elevated temperatures. The technology behind this immobilization technique - here termed ''light assisted immobilization'' - is based on the fact that disulphide bridges that are naturally present within the protein structure can be broken as a result of UV-illumination. The free thiol groups (-SH) created upon disruption of a disulphide bridge are very reactive and can be used as linkers for covalent attachment to a surface. The surface can for example be gold or thiol-derivatized silicon, making this technology extremely useful for a large range of application areas, including biosensors.

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Nanotech 2005 Conference Program Abstract