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Development of Nano-sized Protein Arrays using Block Copolymer Self-Assemblies

J-M Jung, K.Y. Kwon, T-H Ha, B.H. Chung and H-T Jung
Korea Advanced Institute of Science and Technology, KR

Keywords:
block-copolymer lithography, nanoarray

Abstract:
The single molecule approach is potentially of great technological interest as a complementary means to obtain information about protein conformation or molecular interaction. The measurement for molecular interaction in single molecule level requires that the molecule in the question is immobilized by binding to an appropriate surface. To achieve single molecule resolution, we have developed a novel fabrication method of nano-patterned array of bio-molecules with ultra-high density, using self-assembly of block-copolymers(BCP). The periodic spherical-domains of ordered block copolymers were obtained on the bio-compatible substrates. Compared to previous lithographic methods, self-assembly of block-copolymers makes it easier to fabrication of patterns with ~ 10 nanometer feature size and to reduce the number of processing steps. In this work, we have detected a single molecule array using protein conjugated gold nanoparticles in BCP nanostructure. A novel approach for positioning functional biomolecules at defined position to produce ultra-high density biomolecule array below 20 nm resolution was achieved in BCP nanostructure which has hexagonally ordered holes on substrates. In this manner, BCP structure played an important role as confining molecules in single molecule level to a hole avoiding intermolecular interaction or nonspecific binding.

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