Apoptosis Characterization by Flouro-deoxy Glucose and Intracellular Sodium Distribution Using MicroPET/MRI in PC3 Induced Prostate Cancer in Mice Tumor
R. Sharma, R.P. Kline and J.K. Katz
Columbia University, US
prostate tumor, FDG-PET. sodium MRI
Background: Intracellular sodium (IC) and FDG accumulate in premalignant tumor sites. Hypothesis: High FDG and IC accumulation may characterize tumor stages. Objective: To develop rapid noninvasive hybrid microPET/MRI method to characterize PC3 tumor in mice. Methods: Sodium MRI by Inversion recovery pulse sequence on 4.23 T MRI and FDG Conchord microPET estimate and map both IC sodium and FDG at differentiated premalignant and malignant sites. These were confirmed by histology and DNA fragment analysis. Result: Apoptosis, necrosis, carcinoma, sarcoma, neoplasia were confirmed by immunostaining and MRI/PET imaging showed distinct Na and FDG distribution patterns suitable tool for chemosensitivity assay. Conclusion: IC sodium indicates apoptosis and FDG pattern indicates higher glycolysis in both pre-malignant and malignant tumors by rapid imaging.
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Nanotech 2005 Conference Program Abstract