Detecting Drug Screen by Acoustic Wave Sensor

Keywords:
drug screen, acoustic wave sensor, flexural plate wave, SARS

Abstract:
The Severe Acute Respiratory Syndrome (SARS) is induced to the patients pervasively pneumonia and respired exhaustion. In previously study (2), the nucleic acid 318 and 510 of S protein was the crucial fragments to bind angiotensin-converting enzyme 2 (ACE2), which was very recently identified as a functional receptor for the SARS virus. For developing anti-SARS drug in traditional way, it’s usually found out the chemical compound with anti-SARS capability to test tissue or animal with infective SARS virus. In this study, we develop a new method to rapidly and simply screen drugs, which can interfere with the interaction between ACE2 and S protein. Electrostatic attraction and molecular recognition, S protein and hACE2, were employed to the functionalized Flexural Plate Wave (FPW) biosensor via Ni2+ chemical reactions. In our research, FPW sensor was used to distinguish the biomaterial recognizable property between adsorption of biomaterial and drugs precursor. We try to detect an anti-SARS drug by the measurement of frequency shift of FPW sensor due to the variation of weight. In our system, an anti-SARS drug transmit into sensor system with immobilization by S-hACE2 hybrid protein in Fig.1, it would destroy interaction between hACE2 and S protein by blocking S protein activity site. Furthermore, the results obtained by FPW measurements would be compared with the traditional methods such as gene engineering, PCR and western blot. Transfering gene and cloning S protein or hACE2 into Sf-9 insect cell and confirming protein activity were shown as in Fig.2, Fig.3 and Fig.4.

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Nanotech 2005 Conference Program Abstract