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Porosity Measurements of new Lyophilized Chitosan Nanobiomaterials Submitted to a Sterilization Process

A. Larena, D.A. Cáceres, M. Vallet-Regí and J. Peña
E T S Industrial Engineering, ES

Keywords:
chitosan, biomaterials, lyophilization

Abstract:
Chitosan nanoparticles have potential applications as prosthesis biodegradable bone regenerator. In vitro biocompatibility of chitosan has been demonstrated (Larena, Cáceres, De la Piedra, Montero, Vicario, Fuentes, Ibarzábal, Bernabeu and Ribelles, 2004) and MIP porosity measurements were carried out in chitosan membranes (Larena and Cáceres, 2004). However low mesoporosities were found. Lyophilization can improve pore sizes and their number of new chitosan nanobiomaterials based on PEG submitted to sterilization. MIP and BET techniques provides us information about pore sizes. For morphology observation SEM and polarization microscopy are used. Thus, new prosthesis lyophilized and sterilized based on PEG/chitosan nanobiomaterials are been searched and their characteristics measured comparing with non sterilized nanomaterials.

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