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Imaging Ellipsometry: A Powerful Method for Biochip Development, QC and Evaluation

M. Laging, D. Hoenig and A. Eing
Accurion, US

Keywords:
DNA, protein, array, binding kinetics, quality control

Abstract:
Biochip applications nowadays are taking the step from DNA to protein applications. Proteins show a vasting variety concerning their individual chemical behaviour, demanding new techniques regarding chip substrates and immobilization chemistries. Furthermore the use fluorescent labeling as detection method in protein chip experiments will in most cases have to be avoided. Especially concerning the structural aspects of binding events, a label free method will be clearly favoured. Ellipsometry as a well established method in material research has substantially enriched the field of protein research. Its special application known as SPR has widely been accepted as the major tool to characterize the interaction of proteins with all classes of molecules. Taking advantage of the full potential of Imaging Ellipsometry provides us with a powerful tool not only allowing label free kinetic measurements on multiple protein and DNA spots in situ: Imaging Ellipsometry allows the control of every step in the area of biochip experimentation, from development of prototype surfaces to a quality control of all fabricational steps, finally the interaction experiments in a high throughput manner. This talk presents the basics of Imaging Ellipsometry and exemplifying data ranging from QC to kinetics on biochips with the Nanofilm EP3 Imaging Ellipsometer platform.

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