Hetero-bifunctional Poly(ethylene glycol) Modified Gold Nanoparticles as an Intracellular Tracking and Delivery Agent
W. Fu, D. Shenoy, J. Li, C. Crasto, G. Jones, C. Dimarzio, S. Sridhar and M. Amiji
Northeastern University, US
gold nanoparticles, biomedical, PEG
To increase the targeting potential, circulation time, and the flexibility of surface-attached biomedically-relevant ligands on gold nanoparticles, hetero-bifunctional poly(ethylene glycol) (PEG, MW 1,500) was synthesized having a thiol group on one terminus and a reactive functional group on the other. Coumarin, a model fluorescent dye, was conjugated to the PEG spacer and gold nanoparticles were modified with coumarin-PEG-thiol. Surface attachment of coumarin through the PEG spacer decreases the fluorescence quenching effect of gold nanoparticles. The results of cellular cytotoxicity and fluorescence confocal analyses showed that the PEG spacer modified nanoparticles were essentially non-toxic and could be efficiently internalized in the cells within one hour of incubation.
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Nanotech 2005 Conference Program Abstract