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Enhancement of E6 Protein Binding on Binding-Orientation-Sensitive Mixed SAMs Molecules

F.G. Tseng, J.K. Hwang, S.W. Hung, J.M. Chang, T.W. Huang, Y.T. Tseng, H.M. Huang and C.C. Chieng
National Tsing Hua University, TW

Keywords:
mixed SAMs, binding, protein, molecular dynamics simulation, atomic force microscopy

Abstract:
Orientation selectivity and binding enhancement with E6 protein are demonstrated by nano-structured mixed-SAMs systems of different chain lengths. Mixtures of n-alkinethiol SAMs of different chain lengths: 1-Decanethiol (C9) and 1-Hexanethiol (C5) are the examples in present work The demonstrations include the molecular dynamics simulations on the binding energies of a single E6 protein molecule with mixed SAMs molecules and the binding force measurements by Atomic Force Microscope (AFM) for a cluster of E6 protein molecules at different orientations with the same SAMs.

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