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Protein Array Fabricated by Micro-Contact Printing Method for Detection of Marker Protein to Genetically Modified Organisms

W. Lee, S-H Paek and J-W Choi
Sogang Univerisity, KR

Keywords:
micro-contact printing, genetically modified organisms

Abstract:
For the past decade, microarray technology has evolved from genomics to proteomics. This trend offers several advantages. In small volumes, biochemical reactions may not be diffusion-limited and may thus be more efficient; less reagent and sample solution are used, lowering costs per test. Minituarized assays can be done quickly and simultaneously in large numbers; small scales can be the key to the realization of certain type of assays, e.g., where laminar flow in capillaries is required. In this study, micro-contact printing (µCP) technique was applied to the formation of protein pattern. Because of the low cost of fabrication as well as the simplicity of transferring target material to substrates, the µCP technique is versatile and very attractive for diverse application. In µCP method, master and poly(dimethylsiloxane) (PDMS) stamp were fabricated sequentially. The fabricated PDMS stamp was coated with a solution containing target material for patterning, and brought in contact with Au substrate. By using the fragmented antibody, the antibody was immobilized on the Au surface in an oriented way. The formation of pattern was visually investigated with fluorescence microscopy (Leica, Germany). As the concentration of target protein decreased, the fluorescence intensity was also decreased, of which the detection limit was 100 pg/mL of human serum albumin (HSA). The layer formation and pattern feature was maintained throughout this study. Experimental results suggest that the protein array fabricated by the proposed method could be successfully applied to the measurement of marker protein of genetically modified organisms (GMO).

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