Nano Science and Technology InstituteNano Science and Technology Institute
Nano Science and Technology Institute 2005 NSTI Nanotechnology Conference & Trade Show
Nanotech 2005
Bio Nano 2005
Business & Investment
Nano Impact Workshop
Program
Sessions
Sunday
Monday
Tuesday
Wednesday
Thursday
Index of Authors
Index of Keywords
Keynote Presentations
Confirmed Speakers
Participating Companies
Industry Focus Sessions
Nanotech Expo
Special Symposia
Conferences
Sponsors
Exhibitors
Venue 2005
Organization
Press Room
Subscribe
Site Map
Nanotech 2005 At A Glance
Nanotech Proceedings
Nanotechnology Proceedings
Global Partner
nano tech
Supporting Organizations
Nanotech 2005 Supporting Organization
Media Sponsors
Nanotech 2005 Medias Sponsors
Event Contact
696 San Ramon Valley Blvd., Ste. 423
Danville, CA 94526
Ph: (925) 353-5004
Fx: (925) 886-8461
E-mail:
 
 

Liposome Encapsulation of Fluorescent Nanoparticles: Quantum Dots and Silica Nanoparticles

C.-S. Chen, J. Yao and R.A. Durst
Cornell University, US

Keywords:
liposome, nanoencapsulation, quantum dot, silica nanoparticle

Abstract:
Quantum dots (QDs) and silica nanoparticles (SNs) are new classes of fluorescent probes that overcome the limitations encountered by organic fluorophores in bioassay and biological imaging applications. We encapsulated QDs and SNs into liposomes by the reverse-phase evaporation method. Nanoparticle-loaded liposomes were separated from unencapsulated nanoparticles by size exclusion chromatography and their characteristics were investigated. Dual-color, two-photon fluorescence correlation spectroscopy was used to measure the number of nanoparticles inside each liposome. Results indicated that nanoparticle-loaded liposomes were formed and separated from unencapsulated nanoparticles by using Sepharose gel. As expected, fluorescence self-quenching of nanoparticles inside liposomes was not observed. When a 0.8 mM solution of 50 nm QDs was used for liposome preparation, each liposome encapsulated an average of three QDs. However, we could not measure the number of SNs inside each liposome due to the spectral overlap between the SNs and nitrobenzoxadiazol-liposomes. Our work showed that both QDs and SNs were encapsulated into liposomes and it provided a methodology to measure the number of nanoparticles inside each liposome with fluorescence correlation spectroscopy.

Back to Program

Sessions Sunday Monday Tuesday Wednesday Thursday Authors

Nanotech 2005 Conference Program Abstract

 
Gold Sponsors
Nanotech Gold Sponsors
Silver Sponsors
Nanotech Silver Sponsors
Gold Key Sponsors
Nanotech Gold Key Sponsors
Nanotech Ventures Sponsors
Nanotech Ventures Sponsors
Sponsors
Nanotech Sponsors
News Headlines
NSTI Online Community
 
 

© Nano Science and Technology Institute, all rights reserved.
Terms of use | Privacy policy | Contact