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Effect of Length of Biotin Modified n-Alkanethiolates on the Formation of a Self-Assembled Streptavidin-Biotin Monolayer on a Gold Surface

D.-J. Kim, E.C. Cho and K.-K. Koo
Sogang University, KR

Keywords:
SAM, streptavidin, biotin

Abstract:
The molecular recognition between streptavidin and biotin makes the streptavidin-biotin system very useful in a wide range of biotechnological applications. Currently, biotin-modified n-alkanethiolates have been extensively studied to create a wide variety of self-assembled monolayers (SAMs) on gold surfaces. For the formation of optimum monolayer films, an appropriate biotin-terminated molecule should be selected to minimize nonspecific interactions between streptavidin and the modified surface and to maximize specific binding between them. In the present work, various monolayer films with adsorption of biotin-terminated long-chain n-alkanethiolates and of mixtures with 11-mercaptoundecanol (MUOH) from solution onto the gold surfaces were prepared and characterized by optical ellipsometry, contact angle goniometry, X-ray photoelectron spectroscopy (XPS), and scanning tunneling microscopy (STM).

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