Micro-Patterning of Ionic Reservoirs within a Double Bilayer Lipid Membrane to Fabricate a 2D Array of Ion-Channel Switch Based Electrochemical Biosensors
J.M. Sansiñena, C.K. Yee, A. Sapuri, B.I. Swanson, A. Redondo and A.N. Parikh
Los Alamos National Laboratory, US
Keywords: bilayer lipid membrane, onic reservoir, Ion-channel, electrochemical, biosensor, array
We present a simple approach for the design of ionic reservoir arrays within a double phospholipid bilayer to ultimately develop a 2D array of ion-channel switch based electrochemical biosensors. As a first step, a primary bilayer lipid membrane is deposited onto an array of electrodes patterned onto a substrate surface. Subsequently, an array of microvoids is created within the bilayer by a wet photolithographic patterning of phospholipid bilayers using a deep UV light source and a quartz/chrome photomask. To ensure registry, the photomask used to pattern bilayers is designed to match up the microvoids within the primary bilayer with the array of electrodes on the substrate surface. The deposition of a secondary bilayer lipid membrane onto the primary bilayer that spans across the patterned microvoids leads to the formation of the array of ionic reservoirs within the double phospholipid bilayer. This is accomplished using giant unilamellar vesicles and by exploiting membrane electrostatics. The use of ion-channels incorporated into the secondary bilayer that covers the individual ionic reservoirs allows the construction of a 2D array of ion-channel switch based electrochemical biosensors that are able to recognize different target-agents simultaneously.
Nanotech 2004 Conference Technical Program Abstract