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An Chip-based-Instant Protein Micro Array Formation and Detection System

F-G Tseng, C.E. Ho, M.H. Chen, K.Y. Hung, C.J. Su, Y.F. Chen, H.M. Huang and C-C Chieng
National Tsing Hua University, TW

Keywords: protein microarray, stamper, detection, system

Abstract:
This paper proposes a novel protein micro arrayer employing 3-in-1 chip system, instantly delivering hundreds of biosamples in parallel for disease diagnosis. The 3-in-1chip system included: micro filling chip, micro stamper array and micro bio-reaction chip, applicable to high throughput disease diagnosis and drug screening. Capillary force is applied to drive liquids flowing through all the chip system. The filling chip contains hand-fill-in reservoir and sealed with PDMS thin film for protein storage and loading to arrayer. The size uniformity of the printed spots by the stamper array can be within 10% standard deviation. The micro photo sensor array is designed for the fluorescent detection. Two human cancer (HURP and E6) markers have been successfully detected by this micro stamper system. Reproducibility and Surface wettability for micro/nano droplet formation around stamping process are investigated by simulation and experiment in this paper.

Nanotech 2004 Conference Technical Program Abstract

 
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